Improved functional preservation with the protective recovery method:
Additional testing also revealed an improvement in functional responding of neurons in adult brain slices prepared with the protective recovery method. This was assessed using population calcium imaging and high-potassium depolarization in brain slices prepared from 5 month old Thy1-GCaMP3 transgenic mice. Neurons from the protective recovery group exhibited larger calcium transients at moderate stimulation levels and there were far more total numbers of functionally responding neurons. In addition, in the cortex region there were fewer neurons exhibiting elevated fluorescence in the basal condition, indicating fewer dead or damaged neurons.
The Thy1-GCaMP3 transgenic mice were developed in the laboratory of Dr. Guoping Feng. For more info please see the original article (Chen et al., 2012 Neuron).