Improved morphological preservation with the protective recovery method
A direct comparison of the traditional sucrose protective cutting method and the NMDG protective recovery method was performed using 5 month old mature adult mice. Acute brain slices were prepared and IR-DIC images acquired at ~5 min and ~30 min following the slicing procedure to evaluate the extent of morphological preservation. This approach revealed a dramatic improvement with the NMDG protective recovery method, with much less initial neuronal swelling evident at 5 minutes and much reduced neuronal shrinkage at 30 minutes post slicing. Thus, the number of neurons that appeared accessible for patch clamp recordings was greatly increased.