2011 SFN poster videos: GCaMP3 functional imaging

        This video shows live confocal microscopy imaging of acute brain slices prepared from 5 month old Thy1-GCaMP3 transgenic mouse brains comparing the conventional "neuroprotective sucrose aCSF cutting method" versus the innovative "NMDG protective recovery method."  The imaging is focused on superficial layers of the brain slices during basal activity and then with neuronal stimulation by bath application of high potassium aCSF (concentrations as indicated).  Elevated intracellular calcium transients correlate with neuronal firing and are seen as increases in green fluorescence. 

 

The Thy1-GCaMP3 transgenic mice were developed in the laboratory of Guoping Feng.  For more info please see the original article (Chen et al., 2012 Neuron).

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Dentate gyrus granule cell layer
 

Cortex layer V
 

2012 SFN poster-related videos: GCaMP functional imaging (adult/aged brain slice)​​

14 month old mouse - Dentate gyrus

 

Acute brain slices prepared from a 14 month old Thy1-GCaMP3 mouse using choline-HEPES recovery method plus NAC treatment.  Live imaging was performed to demonstrate functional activation with 20 uM and then 50 uM (saturating) NMDA.

 

19 month old mouse - cortex

Acute brain slices prepared from a 19 month old Thy1-GCaMP3 mouse using choline-HEPES recovery method plus NAC treatment.  Live imaging was performed to demonstrate functional activation with +5 mM K+.

Videos